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Journal: iScience
Article Title: Evolution of ion transporter Na + /K + -ATPase expression in the osmoregulatory maxillary glands of an invasive copepod
doi: 10.1016/j.isci.2024.110278
Figure Lengend Snippet: Whole-animal immunolabeled NKA protein expression in E. carolleeae populations, showing evolutionary shifts between saline and freshwater populations and acclimatory shifts across treatment salinities (0, 5, and 15 PSU). (A) Western blot membrane containing freshwater (FW) and saline (SW) populations at each treatment salinity (green bands) and a molecular marker (MM, red bands). (B) Whole-animal NKA protein expression, measured as integrated band signal intensity in saline and freshwater populations across the treatment salinities. Band signal intensity data were cube root transformed and normalized for the size of the animals (total prosome length in μ m) and membrane variation (supermix band signal). Scale of the normalized band signal is measured as (pixel intensity of experimental band)/(pixel intensity of supermix band x total prosome length in μ m). Data are represented as mean ± SE. Level of significance is indicated with asterisks (∗ = p < 0.05). Salinity had a significant effect on Whole-Animal NKA (two-way ANOVA: F = 3.30, df = 2,34, p = 0.0490). A priori comparisons of the two populations at each treatment salinity did reveal that Whole-Animal NKA was higher in saline copepods relative to freshwater copepods at the 15 PSU treatment salinity (Student’s t test; t = −2.17, df = 11 p = 0.050). Population means and standard errors are reported in Table S3 .
Article Snippet: Chameleon® Duo protein size marker , Li-COR , Cat# 928-60000.
Techniques: Immunolabeling, Expressing, Saline, Western Blot, Membrane, Marker, Transformation Assay